mouse anti tfiib Search Results


tfiia  (Bethyl)
90
Bethyl tfiia
Tfiia, supplied by Bethyl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tfiih  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology tfiih
Tfiih, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti tfiib sc274  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology rabbit anti tfiib sc274
Rabbit Anti Tfiib Sc274, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti phosphomek1 2 pmek 4169  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc anti phosphomek1 2 pmek 4169
KEY RESOURCES TABLE
Anti Phosphomek1 2 Pmek 4169, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti tfiib  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology rabbit anti tfiib
KEY RESOURCES TABLE
Rabbit Anti Tfiib, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tfiih p62  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology tfiih p62
FIGURE 3. CDK7 is a CTD Ser-7 kinase. A, shown is an immobilized template assay on the pGL2-MRG5 pro- moter template in the presence of the activator GAL-VP16. Mock-treated or CDK7-, CDK8-, and CDK9-depleted nuclear extracts (lanes 5–8) were used in the PIC formation reactions. Reactions were carried out under stand- ard in vitro transcription conditions in the presence of 1 M ATP. Lanes 1–4 show 20% of reaction input. B, shown is an immobilized template assay on the ML-promoter template in the presence of GAL-VP16 using mock-treated (Iso), <t>core-TFIIH</t> <t>(p62),</t> CDK7 (CDK7)- or CDK8-depleted (CDK8) Jurkat nuclear extract. Lanes 1–4 show 20% reaction input. TXN, in vitro transcription analysis. C, shown is an immobilized template assay on ML-promoter DNA templates under basal conditions at physiological salt concentrations. PICs were formed in the presence of the indicated amounts of ATPS, washed, and then analyzed by immunoblot. D, shown is an immobilized template assay on the ML-promoter DNA template using mock-treated (Iso), Mediator-depleted (MED), and CDK7-depleted (CDK7) 0.5 M P11 fractions together with GAL4-VP16, recombinant yeast TBP, Toa, and recombinant human TFIIB as indicated. In lane 6 a high salt-washed MED15-IP was added. PIC forma- tion was carried out under physiological conditions in the presence of 1 M ATP and 10 M ATPS. NEX, nuclear extract.E,immobilizedtemplateassayonML-promoterDNAtemplatesusingeitherJurkatnuclearextract(lane 1) or a recombinant transcription system consisting of yeast TBP, Toa, human TFIIB, TFIIE, and TFIIF) in combi- nation with a high salt-washed Mediator-IP (which also provides RNAPII; lanes 2 and 3) is shown. In lane 3 a stringently washed CDK7 IP was also added to the PIC formation reactions. PIC formation in D and E was carried out under physiological conditions in the presence of 1 M ATP and 10 M ATPS.
Tfiih P62, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-mouse par-1 antibody s-19  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology anti-mouse par-1 antibody s-19
FIGURE 3. CDK7 is a CTD Ser-7 kinase. A, shown is an immobilized template assay on the pGL2-MRG5 pro- moter template in the presence of the activator GAL-VP16. Mock-treated or CDK7-, CDK8-, and CDK9-depleted nuclear extracts (lanes 5–8) were used in the PIC formation reactions. Reactions were carried out under stand- ard in vitro transcription conditions in the presence of 1 M ATP. Lanes 1–4 show 20% of reaction input. B, shown is an immobilized template assay on the ML-promoter template in the presence of GAL-VP16 using mock-treated (Iso), <t>core-TFIIH</t> <t>(p62),</t> CDK7 (CDK7)- or CDK8-depleted (CDK8) Jurkat nuclear extract. Lanes 1–4 show 20% reaction input. TXN, in vitro transcription analysis. C, shown is an immobilized template assay on ML-promoter DNA templates under basal conditions at physiological salt concentrations. PICs were formed in the presence of the indicated amounts of ATPS, washed, and then analyzed by immunoblot. D, shown is an immobilized template assay on the ML-promoter DNA template using mock-treated (Iso), Mediator-depleted (MED), and CDK7-depleted (CDK7) 0.5 M P11 fractions together with GAL4-VP16, recombinant yeast TBP, Toa, and recombinant human TFIIB as indicated. In lane 6 a high salt-washed MED15-IP was added. PIC forma- tion was carried out under physiological conditions in the presence of 1 M ATP and 10 M ATPS. NEX, nuclear extract.E,immobilizedtemplateassayonML-promoterDNAtemplatesusingeitherJurkatnuclearextract(lane 1) or a recombinant transcription system consisting of yeast TBP, Toa, human TFIIB, TFIIE, and TFIIF) in combi- nation with a high salt-washed Mediator-IP (which also provides RNAPII; lanes 2 and 3) is shown. In lane 3 a stringently washed CDK7 IP was also added to the PIC formation reactions. PIC formation in D and E was carried out under physiological conditions in the presence of 1 M ATP and 10 M ATPS.
Anti Mouse Par 1 Antibody S 19, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tfii  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc tfii
FIGURE 3. CDK7 is a CTD Ser-7 kinase. A, shown is an immobilized template assay on the pGL2-MRG5 pro- moter template in the presence of the activator GAL-VP16. Mock-treated or CDK7-, CDK8-, and CDK9-depleted nuclear extracts (lanes 5–8) were used in the PIC formation reactions. Reactions were carried out under stand- ard in vitro transcription conditions in the presence of 1 M ATP. Lanes 1–4 show 20% of reaction input. B, shown is an immobilized template assay on the ML-promoter template in the presence of GAL-VP16 using mock-treated (Iso), <t>core-TFIIH</t> <t>(p62),</t> CDK7 (CDK7)- or CDK8-depleted (CDK8) Jurkat nuclear extract. Lanes 1–4 show 20% reaction input. TXN, in vitro transcription analysis. C, shown is an immobilized template assay on ML-promoter DNA templates under basal conditions at physiological salt concentrations. PICs were formed in the presence of the indicated amounts of ATPS, washed, and then analyzed by immunoblot. D, shown is an immobilized template assay on the ML-promoter DNA template using mock-treated (Iso), Mediator-depleted (MED), and CDK7-depleted (CDK7) 0.5 M P11 fractions together with GAL4-VP16, recombinant yeast TBP, Toa, and recombinant human TFIIB as indicated. In lane 6 a high salt-washed MED15-IP was added. PIC forma- tion was carried out under physiological conditions in the presence of 1 M ATP and 10 M ATPS. NEX, nuclear extract.E,immobilizedtemplateassayonML-promoterDNAtemplatesusingeitherJurkatnuclearextract(lane 1) or a recombinant transcription system consisting of yeast TBP, Toa, human TFIIB, TFIIE, and TFIIF) in combi- nation with a high salt-washed Mediator-IP (which also provides RNAPII; lanes 2 and 3) is shown. In lane 3 a stringently washed CDK7 IP was also added to the PIC formation reactions. PIC formation in D and E was carried out under physiological conditions in the presence of 1 M ATP and 10 M ATPS.
Tfii, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monoclonal mouse anti-human coup-tfii h7147  (Perseus Proteomics)
90
Perseus Proteomics monoclonal mouse anti-human coup-tfii h7147
FIGURE 3. CDK7 is a CTD Ser-7 kinase. A, shown is an immobilized template assay on the pGL2-MRG5 pro- moter template in the presence of the activator GAL-VP16. Mock-treated or CDK7-, CDK8-, and CDK9-depleted nuclear extracts (lanes 5–8) were used in the PIC formation reactions. Reactions were carried out under stand- ard in vitro transcription conditions in the presence of 1 M ATP. Lanes 1–4 show 20% of reaction input. B, shown is an immobilized template assay on the ML-promoter template in the presence of GAL-VP16 using mock-treated (Iso), <t>core-TFIIH</t> <t>(p62),</t> CDK7 (CDK7)- or CDK8-depleted (CDK8) Jurkat nuclear extract. Lanes 1–4 show 20% reaction input. TXN, in vitro transcription analysis. C, shown is an immobilized template assay on ML-promoter DNA templates under basal conditions at physiological salt concentrations. PICs were formed in the presence of the indicated amounts of ATPS, washed, and then analyzed by immunoblot. D, shown is an immobilized template assay on the ML-promoter DNA template using mock-treated (Iso), Mediator-depleted (MED), and CDK7-depleted (CDK7) 0.5 M P11 fractions together with GAL4-VP16, recombinant yeast TBP, Toa, and recombinant human TFIIB as indicated. In lane 6 a high salt-washed MED15-IP was added. PIC forma- tion was carried out under physiological conditions in the presence of 1 M ATP and 10 M ATPS. NEX, nuclear extract.E,immobilizedtemplateassayonML-promoterDNAtemplatesusingeitherJurkatnuclearextract(lane 1) or a recombinant transcription system consisting of yeast TBP, Toa, human TFIIB, TFIIE, and TFIIF) in combi- nation with a high salt-washed Mediator-IP (which also provides RNAPII; lanes 2 and 3) is shown. In lane 3 a stringently washed CDK7 IP was also added to the PIC formation reactions. PIC formation in D and E was carried out under physiological conditions in the presence of 1 M ATP and 10 M ATPS.
Monoclonal Mouse Anti Human Coup Tfii H7147, supplied by Perseus Proteomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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emerin antibody  (Proteintech)
91
Proteintech emerin antibody
FIGURE 3. CDK7 is a CTD Ser-7 kinase. A, shown is an immobilized template assay on the pGL2-MRG5 pro- moter template in the presence of the activator GAL-VP16. Mock-treated or CDK7-, CDK8-, and CDK9-depleted nuclear extracts (lanes 5–8) were used in the PIC formation reactions. Reactions were carried out under stand- ard in vitro transcription conditions in the presence of 1 M ATP. Lanes 1–4 show 20% of reaction input. B, shown is an immobilized template assay on the ML-promoter template in the presence of GAL-VP16 using mock-treated (Iso), <t>core-TFIIH</t> <t>(p62),</t> CDK7 (CDK7)- or CDK8-depleted (CDK8) Jurkat nuclear extract. Lanes 1–4 show 20% reaction input. TXN, in vitro transcription analysis. C, shown is an immobilized template assay on ML-promoter DNA templates under basal conditions at physiological salt concentrations. PICs were formed in the presence of the indicated amounts of ATPS, washed, and then analyzed by immunoblot. D, shown is an immobilized template assay on the ML-promoter DNA template using mock-treated (Iso), Mediator-depleted (MED), and CDK7-depleted (CDK7) 0.5 M P11 fractions together with GAL4-VP16, recombinant yeast TBP, Toa, and recombinant human TFIIB as indicated. In lane 6 a high salt-washed MED15-IP was added. PIC forma- tion was carried out under physiological conditions in the presence of 1 M ATP and 10 M ATPS. NEX, nuclear extract.E,immobilizedtemplateassayonML-promoterDNAtemplatesusingeitherJurkatnuclearextract(lane 1) or a recombinant transcription system consisting of yeast TBP, Toa, human TFIIB, TFIIE, and TFIIF) in combi- nation with a high salt-washed Mediator-IP (which also provides RNAPII; lanes 2 and 3) is shown. In lane 3 a stringently washed CDK7 IP was also added to the PIC formation reactions. PIC formation in D and E was carried out under physiological conditions in the presence of 1 M ATP and 10 M ATPS.
Emerin Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti mouse tata box binding protein tbp mab  (Proteintech)
93
Proteintech anti mouse tata box binding protein tbp mab
FIGURE 3. CDK7 is a CTD Ser-7 kinase. A, shown is an immobilized template assay on the pGL2-MRG5 pro- moter template in the presence of the activator GAL-VP16. Mock-treated or CDK7-, CDK8-, and CDK9-depleted nuclear extracts (lanes 5–8) were used in the PIC formation reactions. Reactions were carried out under stand- ard in vitro transcription conditions in the presence of 1 M ATP. Lanes 1–4 show 20% of reaction input. B, shown is an immobilized template assay on the ML-promoter template in the presence of GAL-VP16 using mock-treated (Iso), <t>core-TFIIH</t> <t>(p62),</t> CDK7 (CDK7)- or CDK8-depleted (CDK8) Jurkat nuclear extract. Lanes 1–4 show 20% reaction input. TXN, in vitro transcription analysis. C, shown is an immobilized template assay on ML-promoter DNA templates under basal conditions at physiological salt concentrations. PICs were formed in the presence of the indicated amounts of ATPS, washed, and then analyzed by immunoblot. D, shown is an immobilized template assay on the ML-promoter DNA template using mock-treated (Iso), Mediator-depleted (MED), and CDK7-depleted (CDK7) 0.5 M P11 fractions together with GAL4-VP16, recombinant yeast TBP, Toa, and recombinant human TFIIB as indicated. In lane 6 a high salt-washed MED15-IP was added. PIC forma- tion was carried out under physiological conditions in the presence of 1 M ATP and 10 M ATPS. NEX, nuclear extract.E,immobilizedtemplateassayonML-promoterDNAtemplatesusingeitherJurkatnuclearextract(lane 1) or a recombinant transcription system consisting of yeast TBP, Toa, human TFIIB, TFIIE, and TFIIF) in combi- nation with a high salt-washed Mediator-IP (which also provides RNAPII; lanes 2 and 3) is shown. In lane 3 a stringently washed CDK7 IP was also added to the PIC formation reactions. PIC formation in D and E was carried out under physiological conditions in the presence of 1 M ATP and 10 M ATPS.
Anti Mouse Tata Box Binding Protein Tbp Mab, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti tfiid tbp n 12  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology rabbit anti tfiid tbp n 12
FIGURE 3. CDK7 is a CTD Ser-7 kinase. A, shown is an immobilized template assay on the pGL2-MRG5 pro- moter template in the presence of the activator GAL-VP16. Mock-treated or CDK7-, CDK8-, and CDK9-depleted nuclear extracts (lanes 5–8) were used in the PIC formation reactions. Reactions were carried out under stand- ard in vitro transcription conditions in the presence of 1 M ATP. Lanes 1–4 show 20% of reaction input. B, shown is an immobilized template assay on the ML-promoter template in the presence of GAL-VP16 using mock-treated (Iso), <t>core-TFIIH</t> <t>(p62),</t> CDK7 (CDK7)- or CDK8-depleted (CDK8) Jurkat nuclear extract. Lanes 1–4 show 20% reaction input. TXN, in vitro transcription analysis. C, shown is an immobilized template assay on ML-promoter DNA templates under basal conditions at physiological salt concentrations. PICs were formed in the presence of the indicated amounts of ATPS, washed, and then analyzed by immunoblot. D, shown is an immobilized template assay on the ML-promoter DNA template using mock-treated (Iso), Mediator-depleted (MED), and CDK7-depleted (CDK7) 0.5 M P11 fractions together with GAL4-VP16, recombinant yeast TBP, Toa, and recombinant human TFIIB as indicated. In lane 6 a high salt-washed MED15-IP was added. PIC forma- tion was carried out under physiological conditions in the presence of 1 M ATP and 10 M ATPS. NEX, nuclear extract.E,immobilizedtemplateassayonML-promoterDNAtemplatesusingeitherJurkatnuclearextract(lane 1) or a recombinant transcription system consisting of yeast TBP, Toa, human TFIIB, TFIIE, and TFIIF) in combi- nation with a high salt-washed Mediator-IP (which also provides RNAPII; lanes 2 and 3) is shown. In lane 3 a stringently washed CDK7 IP was also added to the PIC formation reactions. PIC formation in D and E was carried out under physiological conditions in the presence of 1 M ATP and 10 M ATPS.
Rabbit Anti Tfiid Tbp N 12, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


KEY RESOURCES TABLE

Journal: Cell

Article Title: Selective activation of PFKL suppresses the phagocytic oxidative burst

doi: 10.1016/j.cell.2021.07.004

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-phosphoMEK1/2 (pMEK) 4169 , Cell Signaling Tech. , 9154S; RRID:AB_2138017.

Techniques: Virus, Cloning, Recombinant, CellTox Assay, Cytotoxicity Assay, Cytokine Assay, Isolation, Activity Assay, Screening Assay, Mass Spectrometry, Software

FIGURE 3. CDK7 is a CTD Ser-7 kinase. A, shown is an immobilized template assay on the pGL2-MRG5 pro- moter template in the presence of the activator GAL-VP16. Mock-treated or CDK7-, CDK8-, and CDK9-depleted nuclear extracts (lanes 5–8) were used in the PIC formation reactions. Reactions were carried out under stand- ard in vitro transcription conditions in the presence of 1 M ATP. Lanes 1–4 show 20% of reaction input. B, shown is an immobilized template assay on the ML-promoter template in the presence of GAL-VP16 using mock-treated (Iso), core-TFIIH (p62), CDK7 (CDK7)- or CDK8-depleted (CDK8) Jurkat nuclear extract. Lanes 1–4 show 20% reaction input. TXN, in vitro transcription analysis. C, shown is an immobilized template assay on ML-promoter DNA templates under basal conditions at physiological salt concentrations. PICs were formed in the presence of the indicated amounts of ATPS, washed, and then analyzed by immunoblot. D, shown is an immobilized template assay on the ML-promoter DNA template using mock-treated (Iso), Mediator-depleted (MED), and CDK7-depleted (CDK7) 0.5 M P11 fractions together with GAL4-VP16, recombinant yeast TBP, Toa, and recombinant human TFIIB as indicated. In lane 6 a high salt-washed MED15-IP was added. PIC forma- tion was carried out under physiological conditions in the presence of 1 M ATP and 10 M ATPS. NEX, nuclear extract.E,immobilizedtemplateassayonML-promoterDNAtemplatesusingeitherJurkatnuclearextract(lane 1) or a recombinant transcription system consisting of yeast TBP, Toa, human TFIIB, TFIIE, and TFIIF) in combi- nation with a high salt-washed Mediator-IP (which also provides RNAPII; lanes 2 and 3) is shown. In lane 3 a stringently washed CDK7 IP was also added to the PIC formation reactions. PIC formation in D and E was carried out under physiological conditions in the presence of 1 M ATP and 10 M ATPS.

Journal: Journal of Biological Chemistry

Article Title: RNA Polymerase II C-terminal Heptarepeat Domain Ser-7 Phosphorylation Is Established in a Mediator-dependent Fashion

doi: 10.1074/jbc.m109.046565

Figure Lengend Snippet: FIGURE 3. CDK7 is a CTD Ser-7 kinase. A, shown is an immobilized template assay on the pGL2-MRG5 pro- moter template in the presence of the activator GAL-VP16. Mock-treated or CDK7-, CDK8-, and CDK9-depleted nuclear extracts (lanes 5–8) were used in the PIC formation reactions. Reactions were carried out under stand- ard in vitro transcription conditions in the presence of 1 M ATP. Lanes 1–4 show 20% of reaction input. B, shown is an immobilized template assay on the ML-promoter template in the presence of GAL-VP16 using mock-treated (Iso), core-TFIIH (p62), CDK7 (CDK7)- or CDK8-depleted (CDK8) Jurkat nuclear extract. Lanes 1–4 show 20% reaction input. TXN, in vitro transcription analysis. C, shown is an immobilized template assay on ML-promoter DNA templates under basal conditions at physiological salt concentrations. PICs were formed in the presence of the indicated amounts of ATPS, washed, and then analyzed by immunoblot. D, shown is an immobilized template assay on the ML-promoter DNA template using mock-treated (Iso), Mediator-depleted (MED), and CDK7-depleted (CDK7) 0.5 M P11 fractions together with GAL4-VP16, recombinant yeast TBP, Toa, and recombinant human TFIIB as indicated. In lane 6 a high salt-washed MED15-IP was added. PIC forma- tion was carried out under physiological conditions in the presence of 1 M ATP and 10 M ATPS. NEX, nuclear extract.E,immobilizedtemplateassayonML-promoterDNAtemplatesusingeitherJurkatnuclearextract(lane 1) or a recombinant transcription system consisting of yeast TBP, Toa, human TFIIB, TFIIE, and TFIIF) in combi- nation with a high salt-washed Mediator-IP (which also provides RNAPII; lanes 2 and 3) is shown. In lane 3 a stringently washed CDK7 IP was also added to the PIC formation reactions. PIC formation in D and E was carried out under physiological conditions in the presence of 1 M ATP and 10 M ATPS.

Article Snippet: Jurkat nuclear extracts were immunodepleted using antiRNAPII (1C7), MED15 (1H7), and MED25 (VC1) rat monoclonal antibodies, anti-TBP, TFIIH p62, CDK7, and CDK9 rabbit polyclonal antibodies (Santa Cruz sc-273, sc-292, sc- 529, and sc-8338, respectively), and anti-TFIIB (IIB8) mouse monoclonal antibodies (Santa Cruz sc-23875) or anti-CDK8 goat polyclonal antibodies (Santa Cruz sc-1521).

Techniques: In Vitro, Western Blot, Recombinant